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Argan oil ameliorates sodium fluoride–induced renal damage via inhibiting oxidative damage, inflammation, and intermediate filament protein expression in male rats

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Abstract

Fluoride is widely distributed in the environment and has been associated with the development of different health hazards in animals and humans. Argan oil (AO) is a natural vegetable oil with various beneficial pharmacological effects. This study was designed to investigate the potential protective effect of AO supplementation as pre-treatment or co-treatment on sodium fluoride (NaF)–induced nephrotoxicity in rats. Male Sprague Dawley rats (n = 50) were randomly assigned to one of five equal groups: control group, AO-treated group (6 ml/kg b.wt.), NaF-treated group (20 mg/kg b.wt.), pre-treated group, and co-treated group. All rats were daily administered by oral gavage for duration of 30 days. The results showed that AO administration significantly improved renal function and antioxidant status and decreased the lipid peroxidation in NaF-treated rats. Additionally, AO normalized the renal levels of inflammatory markers and mRNA expression level of the intermediate filament protein genes, indicating NaF-induced podocyte damage was ameliorated. Histopathological evaluation of the kidney confirmed the before mentioned biochemical results. AO counteracted the nephrotoxic effects of NaF in rats particularly at co-exposure. These results concluded that AO administration exhibited a significant nephroprotective effect against renal injury induced by NaF in rats.

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Authors and Affiliations

  1. Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt

    Taghred M. Saber

  2. Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt

    Mohamed Fouad Mansour

  3. Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt

    Ahmed Shaban Abdelaziz

  4. Department of Pharmacology, Faculty of Medicine, Zagazig University, Zagazig, 44519, Egypt

    Rasha M. S. Mohamed

  5. Department of Biochemistry, Faculty of Medicine, Zagazig University, Zagazig, 44519, Egypt

    Rania A. Fouad

  6. Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt

    Ahmed Hamed Arisha

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  1. Taghred M. Saber
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  2. Mohamed Fouad Mansour
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  3. Ahmed Shaban Abdelaziz
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  4. Rasha M. S. Mohamed
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  5. Rania A. Fouad
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  6. Ahmed Hamed Arisha
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Correspondence to Taghred M. Saber or Ahmed Hamed Arisha.

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All experimental procedures were done according to the National Institutes of Health (NIH) guidelines for the Care and Use of Laboratory Animals. An approval of the current protocol was obtained from the Institutional Animal Care and Use Committee, Zagazig University, Egypt (ZU-IACUC /2 /F /119 /2019).

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Saber, T.M., Mansour, M.F., Abdelaziz, A.S. et al. Argan oil ameliorates sodium fluoride–induced renal damage via inhibiting oxidative damage, inflammation, and intermediate filament protein expression in male rats. Environ Sci Pollut Res 27, 30426–30436 (2020). https://doi.org/10.1007/s11356-020-09366-z

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